Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells.

Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells.

Blog Article

A crucial step in generating de novo immune responses is the polarization of naive cognate CD4+ T cells by pathogen-triggered dendritic cells (DC).In the human setting, standardized DC-dependent systems are lacking to study molecular events during the initiation of a naive CD4+ T cell response.We developed a TCR-restricted assay to compare different pathogen-triggered human DC for their capacities to instruct functional differentiation of autologous, naive CD4+ T cells.We demonstrated that this methodology can be applied to AEG BPK842720M Electric Single Stainless Steel Pyrolytic Oven compare differently matured DC in terms of kinetics, direction, and magnitude of the naive CD4+ T cell response.Furthermore, we showed the applicability of this assay to study the T cell polarizing capacity of low-frequency blood-derived DC populations directly isolated ex vivo.

This methodology for addressing APC-dependent instruction of naive CD4+ T cells in a human autologous setting will Laundry All-in-One provide researchers with a valuable tool to gain more insight into molecular mechanisms occurring in the early phase of T cell polarization.In addition, it may also allow the study of pharmacological agents on DC-dependent T cell polarization in the human system.